Материал: Огурцов А. Н. Методы бииоинформационного анализа

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S2 (BLOSSUM80) = s(G,G) + s(S,S) + s(A,G) + + s(H , H ) + s(K,Q) = =9 +7 −0 −3 +1−4 +9 −2 −1−4 −1−2 −1+10 −3 +6 +

+[−14](пропуск) −1−1−2 +12 +0 −3 +10 +3 −5 −3 +7 −6 + +2 +7 +6 −4 +10 +[−18](пропуск) +0 −1+7 +12 + 2 = 41.

2.6. МОДЕЛЬНЫЕ ВЫРАВНИВАНИЯ

Ниже приведены результаты парного выравнивания, выполненные программой BLAST, при поиске гомологов для заданной аминокислотной последовательности альфа-субъединицы гемоглобина человека

HBA_HUMAN

>sp|P69905|HBA_HUMAN Hemoglobin subunit alpha OS=Homo sapiens GN=HBA1 PE=1 SV=2 MVLSPADKTNVKAAWGKVGAHAGEYGAEALERMFLSFPTTKTYFPHFDLSHGSAQVKGHGKKVADALTNAVAHVDDMPN ALSALSDLHAHKLRVDPVNFKLLSHCLLVTLAAHLPAEFTP

AVHASLDKFLASVSTVLTSKYR

Фрагменты каждого выравнивания, вместе с матрицами замен аминокислот 2–9, будут использованы в задачах по вычислению счёта выравнивания и составления таблицы динамического программирования глобального выравнивания двух последовательностей.

Выравнивание 1 с бэта-2 субъединицей гемоглобина Rattus norvegicus (Серая крыса).

hemoglobin subunit beta-2 [Rattus norvegicus] Length=147

GENE ID: 100134871 LOC100134871 | beta globin minor gene [Rattus norvegicus] (10 or fewer PubMed links)

Score = 115 bits (288), Expect = 1e-24, Method: Compositional matrix adjust. Identities = 63/145 (44%), Positives = 87/145 (60%), Gaps = 8/145 (5%)

Query	3	LSPADKTNVKAAWGKVGAHAGEYGAEALERMFLSFPTTKTYFPHF-DLSHGSA-----QV						56
Sbjct	4	L+ A+K	V	WGKV +A	GAEAL R+ + +P T+ YF F DLS SA		QV	61
		LTDAEKATVSGLWGKV--NADNVGAEALGRLLVVYPWTQRYFSKFGDLSSASAIMGNPQV
Query	57	KGHGKKVADALTNAVAHVDDMPNALSALSDLHAHKLRVDPVNFKLLSHCLLVTLAAHLPA						116
Sbjct	62	K HGKKV +A		+ + H+D++		+ LS+LH KL VDP NF+LL + +++ L	HL	121
		KAHGKKVINAFNDGLKHLDNLKGTFAHLSELHCDKLHVDPENFRLLGNMIVIVLGHHLGK
Query	117	EFTPAVHASLDKFLASVSTVLTSKY				141
Sbjct	122	EFTP	A+	K +A V++ L	KY	146
		EFTPCAQAAFQKVVAGVASALAHKY

Выравнивание 2 с бэта-субъединицей гемоглобина Papio anubis (Павиан догеровский, Анубис).

hemoglobin subunit beta [Papio anubis] Length=147

GENE ID: 100137310 HBB | hemoglobin, beta [Papio anubis] (10 or fewer PubMed links)

Score = 114 bits (284), Expect = 4e-24, Method: Compositional matrix adjust. Identities = 63/145 (44%), Positives = 86/145 (60%), Gaps = 8/145 (5%)

Query	3	LSPADKTNVKAAWGKVGAHAGEYGAEALERMFLSFPTTKTYFPHF-DLSH-----GSAQV						56
Sbjct	4	L+P +K	V A WGKV	+	E G EAL R+ + +P T+ +F F DLS		G+ +V	61
		LTPEEKNAVTALWGKV--NVDEVGGEALGRLLVVYPWTQRFFDSFGDLSSPAAVMGNPKV
Query	57	KGHGKKVADALTNAVAHVDDMPNALSALSDLHAHKLRVDPVNFKLLSHCLLVTLAAHLPA						116
Sbjct	62	K HGKKV	A ++ + H+D++			+ LS+LH KL VDP NFKLL + L+	LA H	121
		KAHGKKVLGAFSDGLNHLDNLKGTFAQLSELHCDKLHVDPENFKLLGNVLVCVLAHHFGK
Query	117	EFTPAVHASLDKFLASVSTVLTSKY				141
Sbjct	122	EFTP V A+ K +A V+		L	KY	146
		EFTPQVQAAYQKVVAGVANALAHKY

Выравнивание 3 с бэта-субъединицей гемоглобина major chain Rattus norvegicus (Серая крыса).

hemoglobin, beta adult major chain [Rattus norvegicus] Length=147

GENE ID: 361619 Hbb-b1 | hemoglobin, beta adult major chain [Rattus norvegicus]

(10 or fewer PubMed links)

Score = 110 bits (275), Expect = 4e-23, Method: Compositional matrix adjust. Identities = 58/145 (40%), Positives = 88/145 (61%), Gaps = 8/145 (5%)

Query	3	LSPADKTNVKAAWGKVGAHAGEYGAEALERMFLSFPTTKTYFPHF-DLSH-----GSAQV						56
Sbjct	4	L+ A+K	V	WGKV +	E GAE+L + + +P T+ YF F DLS		G+ QV	61
		LTDAEKATVNGLWGKV--NPVEIGAESLASLLIVYPWTQRYFSKFGDLSSVSAIMGNPQV
Query	57	KGHGKKVADALTNAVAHVDDMPNALSALSDLHAHKLRVDPVNFKLLSHCLLVTLAAHLPA						116
Sbjct	62	K HG+KV +A		+ + H+D++		++LS+LH KL VDP NF+LL + +++ +	HL	121
		KAHGEKVINAFDDGLKHLDNLKGTFASLSELHCDKLHVDPENFRLLGNMIVIMMGHHLGK
Query	117	EFTPAVHASLDKFLASVSTVLTSKY				141
Sbjct	122	EFTP+	A+	K +A V++ L	KY	146
		EFTPSAQAAFQKVVAGVASALAHKY

Выравнивание 4 с гемоглобином эпсилон Bos taurus (Дикий бык).

hemoglobin, epsilon 1 [Bos taurus]

Length=147

GENE ID: 539994 HBE1 | hemoglobin, epsilon 1 [Bos taurus] (10 or fewer PubMed links)

Score = 106 bits (265), Expect = 5e-22, Method: Compositional matrix adjust. Identities = 57/145 (40%), Positives = 84/145 (58%), Gaps = 8/145 (5%)

Query	3	LSPADKTNVKAAWGKVGAHAGEYGAEALERMFLSFPTTKTYFPHF-DLSHGSA-----QV						56
Sbjct	4	+ +K	+	WGKV	E G EAL R+ + +P T+ +F F +LS SA		+V	61
		FTAEEKAAITGLWGKVNVE--EAGGEALGRLLVVYPWTQRFFDSFGNLSSASAIMGNPKV
Query	57	KGHGKKVADALTNAVAHVDDMPNALSALSDLHAHKLRVDPVNFKLLSHCLLVTLAAHLPA						116
Sbjct	62	K HGKKV	+	A+ ++D++	A + LS+LH KL VDP NF+LL + +++ LA H			121
		KAHGKKVLTSFGEAIKNLDNLKGAFAKLSELHCDKLHVDPENFRLLGNVIVIILATHFGR
Query	117	EFTPAVHASLDKFLASVSTVLTSKY				141
Sbjct	122	EFTP V A+		K ++ V+T L	KY	146
		EFTPDVQAAWQKLVSGVATALAHKY

Выравнивание 5 с субъединицей бэта гемоглобина Ovis aries (Домашняя овца).

hemoglobin subunit beta [Ovis aries] Length=145

GENE ID: 100049064 HBB | hemoglobin, beta [Ovis aries] (10 or fewer PubMed links)

Score = 106 bits (265), Expect = 6e-22, Method: Compositional matrix adjust. Identities = 64/146 (44%), Positives = 88/146 (61%), Gaps = 8/146 (5%)

Query	2	VLSPADKTNVKAAWGKVGAHAGEYGAEALERMFLSFPTTKTYFPHF-DLSH-----GSAQ					55
Sbjct	1	+L+ +K V	WGKV	E GAEAL R+ + +P T+ +F HF DLS		+A+	58
		MLTAEEKAAVTGFWGKV--KVDEVGAEALGRLLVVYPWTQRFFEHFGDLSSADAVMNNAK
Query	56	VKGHGKKVADALTNAVAHVDDMPNALSALSDLHAHKLRVDPVNFKLLSHCLLVTLAAHLP					115
Sbjct	59	VK HGKKV D+ +N V H+DD+			+ LS+LH KL VDP NF+LL + L+V LA H		118
		VKAHGKKVLDSFSNGVQHLDDLKGTFAQLSELHCDKLHVDPENFRLLGNVLVVVLARHHG
Query	116	AEFTPAVHASLDKFLASVSTVLTSKY			141
Sbjct	119	+EFTP + A	K +A V+ L	+Y	144
		SEFTPVLQAEFQKVVAGVANALAHRY

Выравнивание 6 с субъединицей бэта-1 гемоглобина Mus musculus (Мышь домовая).

hemoglobin subunit beta-1 [Mus musculus] Length=147

GENE ID: 15129 Hbb-b1 | hemoglobin, beta adult major chain [Mus musculus] (Over 100 PubMed links)

Score = 106 bits (264), Expect = 7e-22, Method: Compositional matrix adjust. Identities = 62/145 (43%), Positives = 87/145 (60%), Gaps = 8/145 (5%)

Query	3	LSPADKTNVKAAWGKVGAHAGEYGAEALERMFLSFPTTKTYFPHF-DLSH-----GSAQV						56
Sbjct	4	L+ A+K	V	WGKV	+A E G EAL R+ + +P T+ YF F DLS		G+A+V	61
		LTDAEKAAVSGLWGKV--NADEVGGEALGRLLVVYPWTQRYFDSFGDLSSASAIMGNAKV
Query	57	KGHGKKVADALTNAVAHVDDMPNALSALSDLHAHKLRVDPVNFKLLSHCLLVTLAAHLPA						116
Sbjct	62	K HGKKV		A + + H+D +		++LS+LH KL VDP NF+LL + +++ L	HL	121
		KAHGKKVITAFNDGLNHLDSLKGTFASLSELHCDKLHVDPENFRLLGNMIVIVLGHHLGK
Query	117	EFTPAVHASLDKFLASVSTVLTSKY				141
Sbjct	122	+FTPA	A+	K +A V+	L KY	146
		DFTPAAQAAFQKVVAGVAAALAHKY

Выравнивание 7 с субъединицей бэта гемоглобина Gallus gallus (Банкивский петух).

hemoglobin subunit beta [Gallus gallus] Length=147

GENE ID: 396485 HBG2 | hemoglobin, gamma G [Gallus gallus] (Over 10 PubMed links)

Score = 105 bits (263), Expect = 1e-21, Method: Compositional matrix adjust. Identities = 57/141 (41%), Positives = 82/141 (59%), Gaps = 8/141 (5%)

Query	7	DKTNVKAAWGKVGAHAGEYGAEALERMFLSFPTTKTYFPHF-DLSH-----GSAQVKGHG						60
Sbjct	8	+K	+	WGKV + E GAEAL R+ + +P T+ +F F +LS		G+	V+ HG	65
		EKQLITGLWGKV--NVAECGAEALARLLIVYPWTQRFFASFGNLSSPTAILGNPMVRAHG
Query	61	KKVADALTNAVAHVDDMPNALSALSDLHAHKLRVDPVNFKLLSHCLLVTLAAHLPAEFTP						120
Sbjct	66	KKV	+	+AV ++D++ N	S LS+LH KL VDP NF+LL	L++ LAAH	+FTP	125
		KKVLTSFGDAVKNLDNIKNTFSQLSELHCDKLHVDPENFRLLGDILIIVLAAHFSKDFTP
Query	121	AVHASLDKFLASVSTVLTSKY			141
Sbjct	126		A+ K + V+ L KY		146
		ECQAAWQKLVRVVAHALARKY

Выравнивание 8 с субъединицей бэта-1 гемоглобина зародыша Xenopus laevis (Обыкновенная шпорцевая лягушка).

hemoglobin larval subunit beta-1 [Xenopus laevis] Length=147

GENE ID: 379431 hbd | hemoglobin, delta [Xenopus laevis] (10 or fewer PubMed links)

Score = 105 bits (261), Expect = 1e-21, Method: Compositional matrix adjust. Identities = 61/145 (43%), Positives = 86/145 (60%), Gaps = 8/145 (5%)


Query	3	LSPADKTNVKAAWGKVGAHAGEYGAEALERMFLSFPTTKTYFPHF-DLSH-----GSAQV									56
Sbjct	4	LS +K+ + A W KV			G +AL R+ + FP T+ YF F +LS+				G+A+V		61
		LSADEKSAINAVWSKVNIEND--GHDALTRLLVVFPWTQRYFSSFGNLSNVAAISGNAKV
Query	57	KGHGKKVADALTNAVAHVDDMPNALSALSDLHAHKLRVDPVNFKLLSHCLLVTLAAHLPA									116
Sbjct	62	+ HGKKV	A+	++ H+DD+ N LS LS			HA +L VDP NFK L+	L++ LA		L A	121
		RAHGKKVLSAVDESIHHLDDIKNFLSVLSTKHAEELHVDPENFKRLADVLVIVLAGKLGA
Query	117	EFTPAVHASLDKFLASVSTVLTSKY				141
Sbjct	122	FTP V A+ +KF A +			L+ Y	146
		AFTPQVQAAWEKFSAGLVAALSHGY
Выравнивание			9	с субъединицей			ро гемоглобина		Gallus	gallus

(Банкивский петух).

hemoglobin subunit rho [Gallus gallus] Length=147

GENE ID: 419079 HBG1 | hemoglobin, gamma A [Gallus gallus] (10 or fewer PubMed links)

Score = 104 bits (260), Expect = 2e-21, Method: Compositional matrix adjust. Identities = 56/144 (39%), Positives = 84/144 (59%), Gaps = 8/144 (5%)

Query	4	SPADKTNVKAAWGKVGAHAGEYGAEALERMFLSFPTTKTYFPHF-DLSH-----GSAQVK				57
Sbjct	5	S +K	+ + W KV	E GAEAL R+ + +P T+ +F +F +LS	G+ +V+	62
		SAEEKQLITSVWSKVNVE--ECGAEALARLLIVYPWTQRFFDNFGNLSSPTAIIGNPKVR
Query	58	GHGKKVADALTNAVAHVDDMPNALSALSDLHAHKLRVDPVNFKLLSHCLLVTLAAHLPAE				117
		HGKKV	+ AV ++D++ N + LS+LH KL VDP NF+LL + L++ LAAH +

Sbjct	63	AHGKKVLSSFGEAVKNLDNIKNTYAKLSELHCEKLHVDPENFRLLGNILIIVLAAHFTKD 122
Query	118	FTPAVHASLDKFLASVSTVLTSKY	141
Sbjct	123	FTP A K ++ V+ L KY	146
		FTPTCQAVWQKLVSVVAHALAYKY

Выравнивание 10 с субъединицей гамма гемоглобина Oryctolagus cuniculus (Дикий кролик).

hemoglobin subunit gamma [Oryctolagus cuniculus] Length=147

GENE ID: 100328858 HBG2 | G-gamma globin [Oryctolagus cuniculus] (10 or fewer PubMed links)

Score = 104 bits (260), Expect = 2e-21, Method: Compositional matrix adjust. Identities = 59/145 (41%), Positives = 85/145 (59%), Gaps = 8/145 (5%)

Query	3	LSPADKTNVKAAWGKVGAHAGEYGAEALERMFLSFPTTKTYFPHF-DLSHGSA-----QV 56
Sbjct	4	+ +K + + W V	+ GAEAL R+ + +P T+ +F F +LS SA	+V	61
		FTAEEKAAITSTWKLVDVE--DAGAEALGRLLVVYPWTQRFFDSFGNLSSSSAIMGNPKV

Query	57	KGHGKKVADALTNAVAHVDDMPNALSALSDLHAHKLRVDPVNFKLLSHCLLVTLAAHLPA			116
Sbjct	62	K HGKKV	A +AV +VDD+ N	+ LS+LH +L VDP NFKLL + L++ LA +	121
		KAHGKKVLTAFGDAVKNVDDLKNTFAHLSELHCDRLHVDPENFKLLGNVLVIVLAKYFGK
Query	117	EFTPAVHASLDKFLASVSTVLTSKY		141
Sbjct	122	EFTP V ++	K +A V+T L KY	146
		EFTPQVQSAWQKLVAGVATALAHKY

Выравнивание 11 с субъединицей эпсилон-4 гемоглобина Bos taurus (Дикий бык).

hemoglobin subunit epsilon-4 [Bos taurus] Length=147

GENE ID: 513108 HBE4 | hemoglobin, beta, epsilon 4 [Bos taurus] (10 or fewer PubMed links)

Score = 104 bits (259), Expect = 3e-21, Method: Compositional matrix adjust. Identities = 53/145 (37%), Positives = 80/145 (56%), Gaps = 8/145 (5%)

Query	3	LSPADKTNVKAAWGKVGAHAGEYGAEALERMFLSFPTTKTYFPHFDLSH------GSAQV					56
Sbjct	4	+ +K V + W KV		G E+L R+ + +P T+ +F F +		G+ +V	61
		FTTEEKAAVASLWAKVNVEV--VGGESLARLLIVYPWTQRFFDSFGNLYSESAIMGNPKV
Query	57	KGHGKKVADALTNAVAHVDDMPNALSALSDLHAHKLRVDPVNFKLLSHCLLVTLAAHLPA					116
Sbjct	62	K HG+KV ++	NA+ H+DD+		+ LS+LH KL VDP NF+LL + +L+ LA H		121
		KAHGRKVLNSFGNAIEHMDDLKGTFADLSELHCDKLHVDPENFRLLGNMILIVLATHFSK
Query	117	EFTPAVHASLDKFLASVSTVLTSKY			141
Sbjct	122	EFTP + AS K	+V+ L	KY	146
		EFTPQMQASWQKLTNAVANALAHKY

Выравнивание 12 с белком LOC445037 Danio rerio (Данио рерио, аквариумная рыбка).

hypothetical protein LOC445037 [Danio rerio] Length=147

GENE ID: 445037 zgc:92880 | zgc:92880 [Danio rerio] (10 or fewer PubMed links)

Score = 103 bits (258), Expect = 3e-21, Method: Compositional matrix adjust. Identities = 57/144 (40%), Positives = 81/144 (57%), Gaps = 8/144 (5%)

Query	4	SPADKTNVKAAWGKVGAHAGEYGAEALERMFLSFPTTKTYFPHF-DLSHGSA-----QVK 57
Sbjct	5	S +++ + + W K+		+ E G + L R+ + +P T+ YF F DLS SA		+V	62
		SDSERKTIASVWSKI--NVDEIGPQTLARVLVVYPWTQRYFGAFGDLSCASAIMGNPKVS
Query	58	GHGKKVADALTNAVAHVDDMPNALSALSDLHAHKLRVDPVNFKLLSHCLLVTLAAHLPAE					117
Sbjct	63	HGK V	AL AV +VDD+		+ LS LH KL VDP NFKLL+ CL + +A +		122
		EHGKTVLKALEKAVKNVDDIKTTYAQLSQLHCEKLNVDPDNFKLLADCLSIVIATNFGPA
Query	118	FTPAVHASLDKFLASVSTVLTSKY			141
Sbjct	123	F PAV ++	K L+ V	LTS+Y	146
		FNPAVQSTWQKLLSVVVAALTSRY

Выравнивание 13 с субъединицей бэта-С гемоглобина Ovis aries (Домашняя овца).

hemoglobin subunit beta-C [Ovis aries] Length=142

GENE ID: 100134870 LOC100134870 | beta-C globin [Ovis aries] (10 or fewer PubMed links)

Score = 103 bits (258), Expect = 4e-21, Method: Compositional matrix adjust. Identities = 62/141 (44%), Positives = 85/141 (61%), Gaps = 8/141 (5%)

Query	7	DKTNVKAAWGKVGAHAGEYGAEALERMFLSFPTTKTYFPHF-DLSH-----GSAQVKGHG				60
Sbjct	3	+K + W KV	E GAEAL R+ + +P T+ +F HF DLS		G+A+VK HG	60
		NKALITGFWSKV--KVDEVGAEALGRLLVVYPWTQRFFEHFGDLSTADAVLGNAKVKAHG
Query	61	KKVADALTNAVAHVDDMPNALSALSDLHAHKLRVDPVNFKLLSHCLLVTLAAHLPAEFTP				120
Sbjct	61	KKV D+ +N V H+DD+		+ LS+LH KL VDP NF+LL + L+V LA H EFTP		120
		KKVLDSFSNGVQHLDDLKGTFAQLSELHCDKLHVDPENFRLLGNVLVVVLARHFGKEFTP
Query	121	AVHASLDKFLASVSTVLTSKY		141
Sbjct	121	+ A K +A V++ L	+Y	141
		ELQAEFQKVVAGVASALAHRY

Выравнивание 14 с субъединицей гамма-1 гемоглобина Papio anubis (Павиан догеровский, Анубис).

hemoglobin subunit gamma-1 [Papio anubis] Length=147

GENE ID: 100137314 HBG1 | hemoglobin, gamma A [Papio anubis]

Score = 103 bits (257), Expect = 5e-21, Method: Compositional matrix adjust. Identities = 55/145 (38%), Positives = 85/145 (59%), Gaps = 8/145 (5%)

Query	3	LSPADKTNVKAAWGKVGAHAGEYGAEALERMFLSFPTTKTYFPHF-DLSH-----GSAQV						56
Sbjct	4	+ +K	V + W K+		E G EAL R+ + +P T+ +F F +LS		G+ +V	61
		FTAEEKAAVTSLWSKINVE--ETGGEALGRLLVVYPWTQRFFDSFGNLSSPSAILGNPKV
Query	57	KGHGKKVADALTNAVAHVDDMPNALSALSDLHAHKLRVDPVNFKLLSHCLLVTLAAHLPA						116
Sbjct	62	K HGKKV	+	+A+ ++D++	A + LS+LH KL VDP NFKLL + +++ LA H			121
		KAHGKKVLTSFGDAIKNMDNLKTAFAKLSELHCDKLHVDPENFKLLGNVMVIILATHFGR
Query	117	EFTPAVHASLDKFLASVSTVLTSKY				141
Sbjct	122	EFTP V A+		K +++V+ L	KY	146
		EFTPEVQAAWQKLVSAVAIALAHKY

Выравнивание 15 с гемоглобином дэльта Xenopus (Silurana) tropicalis

(Водная лягушка силураны).

hemoglobin, delta [Xenopus (Silurana) tropicalis] Length=147

GENE ID: 448665 hbd | hemoglobin, delta [Xenopus (Silurana) tropicalis] (10 or fewer PubMed links)

Score = 103 bits (257), Expect = 5e-21, Method: Compositional matrix adjust. Identities = 61/145 (43%), Positives = 85/145 (59%), Gaps = 8/145 (5%)

Query	3	LSPADKTNVKAAWGKVGAHAGEYGAEALERMFLSFPTTKTYFPHF-DLSH-----GSAQV				56
Sbjct	4	LS +K+ + A W KV	G EAL R+ + +P T+ YF F +LS+		G+A+V	61
		LSADEKSAINAVWQKVDIEKD--GHEALTRLLVVYPWTQRYFSSFGNLSNVTAISGNAKV
Query	57	KGHGKKVADALTNAVAHVDDMPNALSALSDLHAHKLRVDPVNFKLLSHCLLVTLAAHLPA				116
Sbjct	62	GHGKKV A+ A+ H+DD+ + LSALS HA +L VDP NFK L+ L + +A			L	121
		HGHGKKVLSAVDEAIHHIDDIKHFLSALSKKHAQELHVDPENFKRLADVLAIVVAGKLGT
Query	117	EFTPAVHASLDKFLASVSTVLTSKY		141
Sbjct	122	FTP V A+ +KF A +	L+ Y	146
		AFTPQVQAAWEKFSAGLVAALSHGY

Выравнивание 16 с субъединицей бэта гемоглобина Bos taurus (Дикий бык).

hemoglobin subunit beta [Bos taurus] Length=145

GENE ID: 280813 HBB | hemoglobin, beta [Bos taurus] (10 or fewer PubMed links)

Score = 101 bits (252), Expect = 2e-20, Method: Compositional matrix adjust. Identities = 63/146 (44%), Positives = 86/146 (59%), Gaps = 8/146 (5%)

Query	2	VLSPADKTNVKAAWGKVGAHAGEYGAEALERMFLSFPTTKTYFPHF-DLSHGSA-----Q					55
Sbjct	1	+L+ +K	V A WGKV	E G EAL R+ + +P T+ +F F DLS A		+	58
		MLTAEEKAAVTAFWGKV--KVDEVGGEALGRLLVVYPWTQRFFESFGDLSTADAVMNNPK
Query	56	VKGHGKKVADALTNAVAHVDDMPNALSALSDLHAHKLRVDPVNFKLLSHCLLVTLAAHLP					115
Sbjct	59	VK HGKKV D+ +N + H+DD+			+ALS+LH KL VDP NFKLL + L+V LA +		118
		VKAHGKKVLDSFSNGMKHLDDLKGTFAALSELHCDKLHVDPENFKLLGNVLVVVLARNFG
Query	116	AEFTPAVHASLDKFLASVSTVLTSKY			141
Sbjct	119	EFTP + A	K +A V+ L	+Y	144
		KEFTPVLQADFQKVVAGVANALAHRY

Выравнивание 17 с субъединицей эпсилон гемоглобина Pan troglodytes (Обыкновенный шимпанзе).

hemoglobin subunit epsilon [Pan troglodytes] Length=147

GENE ID: 100190972 HBE1 | hemoglobin, epsilon 1 [Pan troglodytes] (10 or fewer PubMed links)

Score = 101 bits (252), Expect = 2e-20, Method: Compositional matrix adjust. Identities = 55/141 (40%), Positives = 84/141 (60%), Gaps = 8/141 (5%)

Query	7	DKTNVKAAWGKVGAHAGEYGAEALERMFLSFPTTKTYFPHF-DLSH-----GSAQVKGHG					60
Sbjct	8	+K V + W K+		E G EAL R+ + +P T+ +F F +LS		G+ +VK HG	65
		EKAAVTSLWSKMNVE--EAGGEALGRLLVVYPWTQRFFDSFGNLSSPSAILGNPKVKAHG
Query	61	KKVADALTNAVAHVDDMPNALSALSDLHAHKLRVDPVNFKLLSHCLLVTLAAHLPAEFTP					120
Sbjct	66	KKV	+ +A+ ++D++	A + LS+LH KL VDP NFKLL + +++ LA H EFTP			125
		KKVLTSFGDAIKNMDNLKPAFAKLSELHCDKLHVDPENFKLLGNVMVIILATHFGKEFTP
Query	121	AVHASLDKFLASVSTVLTSKY			141
Sbjct	126	V A+	K +++V+ L	KY	146
		EVQAAWQKLVSAVAIALAHKY

Выравнивание 18 с субъединицей эпсилон гемоглобина Gallus gallus (Банкивский петух).

hemoglobin subunit epsilon [Gallus gallus] Length=147

GENE ID: 428115 HBE | hemoglobin, beta [Gallus gallus] (10 or fewer PubMed links)

Score = 101 bits (251), Expect = 2e-20, Method: Compositional matrix adjust. Identities = 55/144 (39%), Positives = 82/144 (57%), Gaps = 8/144 (5%)


Query	4	SPADKTNVKAAWGKVGAHAGEYGAEALERMFLSFPTTKTYFPHF-DLSH-----GSAQVK					57
Sbjct	5	S +K	+ + W KV	E GAEAL R+ + +P T+ +F F +LS		G+ +V+	62
		SAEEKQLITSVWSKVNVE--ECGAEALARLLIVYPWTQRFFASFGNLSSPTAIMGNPRVR
Query	58	GHGKKVADALTNAVAHVDDMPNALSALSDLHAHKLRVDPVNFKLLSHCLLVTLAAHLPAE					117
Sbjct	63	HGKKV	+ AV ++D++ N		+ LS+LH KL VDP NF+LL	L++ LA+H +	122
		AHGKKVLSSFGEAVKNLDNIKNTYAKLSELHCDKLHVDPENFRLLGDILIIVLASHFARD
Query	118	FTPAVHASLDKFLASVSTVLTSKY			141
Sbjct	123	FTPA	+ K + V+ L KY		146
		FTPACQFAWQKLVNVVAHALARKY

Выравнивание 19 с белком LOC100216007 Xenopus (Silurana) tropicalis

(Водная лягушка силураны).

hypothetical protein LOC100216007 [Xenopus (Silurana) tropicalis] Length=147

GENE ID: 100216007 LOC100216007 | hypothetical protein LOC100216007 [Xenopus (Silurana) tropicalis] (10 or fewer PubMed links)

Score = 101 bits (251), Expect = 3e-20, Method: Compositional matrix adjust. Identities = 55/142 (39%), Positives = 83/142 (59%), Gaps = 8/142 (5%)

Query	7	DKTNVKAAWGKVGAHAGEYGAEALERMFLSFPTTKTYFPHF-DLSH-----GSAQVKGHG 60
Sbjct	8	+K + + W KV	+ G EAL R+ + +P T+ YF F +LS+	G+A+V+ HG	65
		EKAAITSVWQKVNLE--QDGHEALTRLLVVYPWTQRYFSSFGNLSNVTAVSGNAKVRAHG

Query	61	KKVADALTNAVAHVDDMPNALSALSDLHAHKLRVDPVNFKLLSHCLLVTLAAHLPAEFTP				120
Sbjct	66	KV	A+ NA+AH+D++	L	LS+ HA KL VDP NFK L L++ LA+ L FTP	125
		NKVLSAVGNAIAHLDNVKGTLHDLSETHAFKLHVDPENFKRLGEVLVIVLASKLGTAFTP
Query	121	AVHASLDKFLASVSTVLTSKYR			142
Sbjct	126	+	+ +KF+A + L+	Y	147
		QIQGAWEKFVAVLVDALSQGYN

Выравнивание 20 с субъединицей эпсилон гемоглобина Macaca mulatta (Макак-резус).

hemoglobin subunit epsilon [Macaca mulatta] Length=147

GENE ID: 715414 HBE1 | hemoglobin, epsilon 1 [Macaca mulatta] (10 or fewer PubMed links)

Score = 100 bits (248), Expect = 5e-20, Method: Compositional matrix adjust. Identities = 54/145 (38%), Positives = 84/145 (58%), Gaps = 8/145 (5%)

Query	3	LSPADKTNVKAAWGKVGAHAGEYGAEALERMFLSFPTTKTYFPHF-DLSH-----GSAQV						56
Sbjct	4	+ +K	V + W K+		E G EAL R+ + +P T+ +F F +LS		G+ +V	61
		FTAEEKAAVTSLWSKMNVE--ETGGEALGRLLVVYPWTQRFFDSFGNLSSPSAILGNPKV
Query	57	KGHGKKVADALTNAVAHVDDMPNALSALSDLHAHKLRVDPVNFKLLSHCLLVTLAAHLPA						116
Sbjct	62	K HGKKV	+	+A+ ++D++		+ LS+LH KL VDP NFKLL + +++ LA H		121
		KAHGKKVLTSFGDAIKNMDNLKITFAKLSELHCDKLHVDPENFKLLGNVMVIILATHFGK
Query	117	EFTPAVHASLDKFLASVSTVLTSKY				141
Sbjct	122	EFTP V A+		K +++V+ L	KY	146
		EFTPEVQAAWQKLVSAVAIALAHKY

Выравнивание 21 с субъединицей бэта-2 гемоглобина Xenopus laevis (Обыкновенная шпорцевая лягушка).

hemoglobin subunit beta-2 [Xenopus laevis] Length=147

GENE ID: 397871 hbg2-b | hemoglobin, gamma G [Xenopus laevis] (10 or fewer PubMed links)

Score = 99.8 bits (247), Expect = 7e-20, Method: Compositional matrix adjust. Identities = 54/142 (39%), Positives = 84/142 (60%), Gaps = 8/142 (5%)


Query	7	DKTNVKAAWGKVGAHAGEYGAEALERMFLSFPTTKTYFPHF-DLSH-----GSAQVKGHG					60
Sbjct	8	+K	+ + W KV	G +AL R+ + +P T+ YF +F +LS+		G+A+V+ HG	65
		EKAAITSVWQKVNVE--HDGHDALGRLLIVYPWTQRYFSNFGNLSNSAAVAGNAKVQAHG
Query	61	KKVADALTNAVAHVDDMPNALSALSDLHAHKLRVDPVNFKLLSHCLLVTLAAHLPAEFTP					120
Sbjct	66	KKV	A+ NA++H+D + ++L LS +HA +L VDP NFK		L++ L A L FTP		125
		KKVLSAVGNAISHIDSVKSSLQQLSKIHATELFVDPENFKRFGGVLVIVLGAKLGTAFTP

Смотрите также:


«ДОХОДЫ, РАСХОДЫ И ПРИБЫЛЬ КОММЕРЧЕСКОГО БАНКА.»
«ДОХОДЫ, РАСХОДЫ И ПРИБЫЛЬ КОММЕРЧЕСКОГО БАНКА.»
Значение, сущность и содержание социально — педагогической деятельности в организации для детей-сирот и детей, оставшихся без попечения родителей
Проактивные методы PR-деятельности российских авиационных компаний «Россия», «Азимут»
__RGR2
__RGR2
_10_Эмиль Золя для эл версии
_11_А. Франс для эл версии
_3 тема - Диффузия