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DICOM PS3.3 2020a - Information Object Definitions​

Page 921​

Attribute Name​

Tag​

Type​

Attribute Description​

 

Number of Focal Planes​

(0048,0013)​

1C​

Number of acquisition focal planes used for extended depth of field.​

 

 

 

Required if Extended Depth of Field (0048,0012) value is YES​

 

 

 

 

Note​

 

 

 

 

 

Total Pixel Matrix Focal Planes (0048,0303) describes the​

 

 

 

 

number of focal planes separately encoded, and is distinct from​

 

 

 

 

Number of Focal Planes (0048,0013), which describes in what​

 

 

 

 

manner different focal planes were combined into a single​

 

 

 

 

encoded plane (focus stacking).​

 

Distance Between Focal​

(0048,0014)​

1C​

Distance between acquisition focal planes used for extended depth of​

Planes​

 

 

field, in µm.​

 

 

 

 

Required if Extended Depth of Field (0048,0012) value is YES.​

 

 

 

 

Note​

 

 

 

 

 

Spacing Between Slices (0018,0088) describes the spacing of​

 

 

 

 

focal planes separately encoded, and is distinct from Distance​

 

 

 

 

Between Focal Planes (0048,0014), which describes in what​

 

 

 

 

manner different focal planes were combined into a single​

 

 

 

 

encoded plane (focus stacking).​

 

Acquisition Device​

(0018,1400)​

3​

Description of visual processing performed on the image prior to​

Processing Description​

 

 

exchange. Examples of this processing are: edge enhanced, gamma​

 

 

 

corrected, convolved (spatially filtered)​

 

Convolution Kernel​

(0018,1210)​

3​

Label for convolution kernel used in acquisition device visual processing​

RecommendedAbsentPixel​

(0048,0015)​

3​

A color value with which it is recommended to display the pixels of the​

CIELab Value​

 

 

Total Pixel Matrix that are not encoded. The units are specified in​

 

 

 

PCS-Values,andthevalueisencodedasCIELab.SeeSectionC.10.7.1.1.​

C.8.12.4.1 Whole Slide Microscopy Image Attribute Descriptions​

C.8.12.4.1.1 Image Type​

Image Type (0008,0008) is specified to be Type 1 with the following constraints:​

Value 1 shall have a value of ORIGINAL or DERIVED​

Value 2 shall have a value of PRIMARY​

Value 3 (Image Flavor) shall have the Defined Terms in Table C.8.12.4-2.​

 

Table C.8.12.4-2. Whole Slide Microscopy Image Flavors​

LOCALIZER​

Collectedforthepurposeofplanningornavigatingotherimages;providesframe-levelnavigationalfeatures.​

VOLUME​

Set of frames that define a regularly sampled volume​

LABEL​

Purpose of image is to capture the slide label only; non-label area shall not be present; the label is shown​

 

in Figure C.8-16.​

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DICOM PS3.3 2020a - Information Object Definitions​

OVERVIEW​

Purpose of image is to provide an overview of the entire glass slide; shall include the label, the specimen​

 

and the empty space that are shown in Figure C.8-16.​

 

Note​

 

One purpose of the OVERVIEW image is to allow visual quality control to assure that tissue​

 

fragments have not been omitted when selecting areas (manually or automatically) to digitize at​

 

high resolution. The OVERVIEW image is distinct from the lowest resolution VOLUME image(s)​

 

of WSI pyramids.​

Value 4 (Derived Pixel) shall have the Defined Terms specified in Table C.8.12.4-3.​

 

Table C.8.12.4-3. Whole Slide Microscopy Image Derived Pixels​

NONE​

No derivation of pixels (original)​

RESAMPLED​

Pixels were derived by down sampling a higher resolution image​

No additional values shall be present.​

C.8.12.4.1.2 Imaged Volume Width, Height, Depth​

ThefullphysicalextentofthewholeslideimagetargetvolumeisdescribedintheAttributesImagedVolumeWidth(0048,0001),Imaged​ Volume Height (0048,0002), and Imaged Volume Depth (0048,0003). These values reflect the maximum extent in these dimensions.​ There is no requirement that this entire extent is actually encoded in frames of the Image SOP Instance, unless Dimension Organiz-​ ation Type (0020,9311) is present with a value of TILED_FULL.​

The value of Imaged Volume Depth (0048,0003) shall not be 0. If only a single focal plane is imaged, the Imaged Volume Depth may​ be the optical depth of field as encoded in Slice Thickness (0018,0050) of the Pixel Measures Functional Group (see Sec-​ tion C.7.6.16.2.1).​

C.8.12.4.1.3 Total Pixel Matrix Columns, Rows, Focal Planes​

TotalPixelMatrixColumns(0048,0006),TotalPixelMatrixRows(0048,0007)andTotalPixelMatrixFocalPlanes(0048,0303)describe​ the size of the entire imaged volume as a single extent across all frames (tiles).​

The extent would be as described in these Attributes if the whole volume would be imaged and encoded as a non-sparse pixel matrix​ with the pixel spacing as specified in Pixel Spacing (0028,0030) of the Pixel Measures Functional Group (see Section C.7.6.16.2.1),​ such as when Dimension Organization Type (0020,9311) is present with a value of TILED_FULL.​

C.8.12.4.1.4 Total Pixel Matrix Origin Sequence and Image Orientation (Slide)​

TotalPixelMatrixOriginSequence(0048,0008)specifiesthelocationofthetopleftmostpixelofthepixelmatrix,andImageOrientation​ (Slide) (0048,0102) specifies the direction cosines of the first row and the first column of the pixel matrix, both with respect to the​ Slide Coordinate System Frame of Reference (see Section C.8.12.2). Although the image acquisition may vary the true row and​ column orientation at the pixel scale to account for local variation in the physical specimen, this Attribute describes the orientation as​ if the Pixel Matrix were flat.​

Note​

Typically, Image Orientation (Slide) will describe only a planar rotation, as the image plane is usually nominally parallel to​ the slide surface.​

C.8.12.4.1.5 Photometric Interpretation and Samples Per Pixel​

See Section C.7.6.3.1.2.​

Enumerated Values for Photometric Interpretation (0028,0004):​

MONOCHROME2​

RGB​

YBR_FULL_422​

YBR_ICT​

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DICOM PS3.3 2020a - Information Object Definitions​

Page 923​

YBR_RCT​

The value shall be appropriate to the compression Transfer Syntax used, if any, and shall be MONOCHROME2 or RGB for uncom-​ pressed or lossless compressed Transfer Syntaxes that do not have defined color space transformations, YBR_ICT or RGB for irre-​ versible JPEG 2000 Transfer Syntaxes, YBR_RCT or RGB for reversible JPEG 2000 Transfer Syntaxes, and YBR_FULL_422 or​ RGB for JPEG lossy compressed Transfer Syntaxes.​

Note​

1.​Future lossless and lossy Transfer Syntaxes may lead to the need for new definitions and choices for Photometric Inter-​ pretation. The Enumerated Values may therefore be extended with additional Photometric Interpretation values directly​ associated with new Transfer Syntaxes that are negotiated, and hence do not render existing implementations non-​ conformant.​

2.​Motion compression Transfer Syntaxes are not expected to be used for Whole Slide Imaging, so the use of​ YBR_PARTIAL_420 for MPEG2, MPEG-4 AVC/H.264 and HEVC/H.265 Transfer Syntaxes is not permitted.​

3.​TheuseofRGBwithJPEGandJPEG2000isonlyintendedtoallowforconversiontoDICOMofimagesfromproprietary​ formats for which no color transformation from RGB to YBR was performed and the encoded components really are​ RGB. The value of Photometric Interpretation (0028,0004) describes what is actually encoded, so a value of RGB is​ not used when the compressed components are actually YBR.​

Multi-spectral images may be encoded as a single wavelength band (color) in each frame using MONOCHROME2, or with up to three​ bands in each frame using one of the color Photometric Interpretations.​

Enumerated Values for Samples per Pixel (0028,0002) when Photometric Interpretation (0028,0004) is MONOCHROME2:​

1​

Enumerated Values for Samples per Pixel (0028,0002) when Photometric Interpretation (0028,0004) is not MONOCHROME2:​

3​

C.8.12.5 Optical Path Module​

TableC.8.12.5-1specifiestheAttributesoftheopticalpathsusedintheVLimaging.Eachopticalpathisacombinationofillumination,​ filters, lenses, and sensors, and each combination is identified for possible reference by Attributes in other Modules. Additional optical​ path parameters may be specified in the Acquisition Context Module.​

Table C.8.12.5-1. Optical Path Module Attributes​

Attribute Name​

Tag​

Type​

Attribute Description​

Number of Optical Paths​

(0048,0302)​

1C​

Number of Items in the Optical Path Sequence (0048,0105).​

 

 

 

RequiredifDimensionOrganizationType(0020,9311)ispresent​

 

 

 

with a value of TILED_FULL. May be present otherwise.​

Optical Path Sequence​

(0048,0105)​

1​

Describes the optical paths used during the acquisition of this​

 

 

 

image.​

 

 

 

One or more Items shall be included in this Sequence.​

 

 

 

See Section C.8.12.5.1.1​

>Optical Path Identifier​

(0048,0106)​

1​

Identifier for the opticalpath specified intheSequence Item. The​

 

 

 

identifier shall be unique for each Item within the Optical Path​

 

 

 

Sequence.​

>Optical Path Description​

(0048,0107)​

3​

Description of the optical path specified in the Sequence Item.​

>Illuminator Type Code​

(0048,0100)​

3​

Type of illuminator.​

Sequence​

 

 

Only a single Item is permitted in this Sequence.​

 

 

 

>>Include Table 8.8-1 “Code Sequence Macro Attributes”​

CID may be defined in the IOD constraints.​

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DICOM PS3.3 2020a - Information Object Definitions​

Attribute Name​

Tag​

Type​

Attribute Description​

>Illumination Wave Length​

(0022,0055)​

1C​

Nominal wavelength of the illuminator in nm. Required if​

 

 

 

Illumination Color Code Sequence (0048,ee08) is not present.​

 

 

 

May be present otherwise.​

>Illumination Color Code​

(0048,0108)​

1C​

Color of the illuminator.​

Sequence​

 

 

Only a single Item shall be included in this Sequence.​

 

 

 

 

 

 

RequiredifIlluminationWaveLength(0022,0055)isnotpresent.​

 

 

 

May be present otherwise.​

>>Include Table 8.8-1 “Code Sequence Macro Attributes”​

CID may be defined in the IOD constraints.​

>Illumination Type Code​

(0022,0016)​

1​

Coded value for illumination method. See Section C.8.12.5.1.2.​

Sequence​

 

 

One or more Items shall be included in this Sequence.​

 

 

 

>>Include Table 8.8-1 “Code Sequence Macro Attributes”​

CID may be defined in the IOD constraints.​

>Light Path Filter Type Stack​

(0022,0017)​

3​

Filters used in the light source (excitation) path.​

Code Sequence​

 

 

One or more Items are permitted in this Sequence.​

 

 

 

 

 

 

See Section C.8.12.5.1.3.​

>>Include Table 8.8-1 “Code Sequence Macro Attributes”​

CID may be defined in the IOD constraints.​

>Light Path Filter​

(0022,0001)​

3​

Nominal pass-through wavelength of light path filter(s) in nm​

Pass-Through Wavelength​

 

 

 

>Light Path Filter Pass Band​

(0022,0002)​

3​

Pass band of light path filter(s) in nm. This Attribute has two​

 

 

 

Values. The first is the shorter and the second the longer​

 

 

 

wavelength relative to the peak. The values are for the - 3dB​

 

 

 

nominal (1/2 of peak) pass through intensity.​

 

 

 

One of the two Values may be zero length, in which case it is a​

 

 

 

cutoff filter.​

>Image Path Filter Type​

(0022,0018)​

3​

Describesstackoffiltersusedinimage(emission)pathbetween​

Stack Code Sequence​

 

 

the imaging target and the optical sensor.​

 

 

 

One or more Items are permitted in this Sequence.​

 

 

 

See Section C.8.12.5.1.3.​

>>Include Table 8.8-1 “Code Sequence Macro Attributes”​

CID may be defined in the IOD constraints.​

>Image Path Filter​

(0022,0003)​

3​

Nominal pass-through wavelength of image path filter(s) in nm​

Pass-Through Wavelength​

 

 

 

>ImagePathFilterPassBand​

(0022,0004)​

3​

Pass band of image path filter(s) in nm. This Attribute has two​

 

 

 

Values. The first is the shorter and the second the longer​

 

 

 

wavelength relative to the peak. The values are for the - 3dB​

 

 

 

nominal (1/2 of peak) pass through intensity.​

 

 

 

One of the two Values may be zero length, in which case it is a​

 

 

 

cutoff filter​

>Lenses Code Sequence​

(0022,0019)​

3​

Lenses that were used in this optical path.​

 

 

 

One or more Items are permitted in this Sequence.​

>>Include Table 8.8-1 “Code Sequence Macro Attributes”​

CID may be defined in the IOD constraints.​

>Condenser Lens Power​

(0048,0111)​

3​

Nominal power of the condenser lens​

>Objective Lens Power​

(0048,0112)​

3​

Nominal power of the objective lens. If a single lens is used as​

 

 

 

both condenser and objective, its power is specified in this​

 

 

 

Attribute.​

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DICOM PS3.3 2020a - Information Object Definitions​

Page 925​

Attribute Name​

Tag​

Type​

Attribute Description​

 

>Objective Lens Numerical​

(0048,0113)​

3​

Nominal numerical aperture of the objective lens.​

 

Aperture​

 

 

 

 

>Channel Description Code​

(0022,001A)​

1C​

Describes the light color sensed for each channel to generate​

Sequence​

 

 

the image.​

 

 

 

 

Required if this differs from the natural interpretation.​

 

 

 

Note​

 

 

 

 

1.​ForMONOCHROME2,thenaturalinterpretationis​

 

 

 

thefullvisiblelightspectrum.Afullspectrumsensor​

 

 

 

may be presented with light of only a single color​

 

 

 

based on illumination and filters.​

 

 

 

 

2.​Equipment may use a color Photometric​

 

 

 

 

Interpretation (RGB, YBR) as a container​

 

 

 

 

representing up to 3 channels of any detected​

 

 

 

wavelength.​

 

 

 

 

Shall have the same number of Items as the value of Samples​

 

 

 

per Pixel Used (0028,0003) if present, or otherwise the value of​

 

 

 

SamplesperPixel(0028,0002).Thechannelsshallbedescribed​

 

 

 

in the order in which the channels are encoded.​

 

>>Include Table 8.8-1 “Code Sequence Macro Attributes”​

CID may be defined in the IOD constraints.​

 

>Palette Color Lookup Table​

(0048,0120)​

3​

For MONOCHROME2 images, provides a transform of the pixel​

Sequence​

 

 

values with this optical path into an RGB color representation.​

 

 

 

The LUT provides a mapping to an appropriate color or​

 

 

 

pseudo-color for display purposes.​

 

 

 

 

Only a single Item is permitted in this Sequence.​

 

>>Include Table C.7-22a “Palette Color Lookup Table Macro​

 

 

Attributes”​

 

 

 

 

>ICC Profile​

(0028,2000)​

1C​

An ICC Profile encoding the transformation of device-dependent​

 

 

 

stored pixel values into PCS-Values. See Section C.8.12.5.1.4​

 

 

 

Required if Photometric Interpretation (0028,0004) is not​

 

 

 

MONOCHROME2, or if Palette Color Lookup Table Sequence​

 

 

 

(0048,0120) is present.​

 

>Color Space​

(0028,2002)​

3​

A label that identifies the well-known color space of the image.​

 

 

 

Shall be consistent with any ICC Profile (0028,2000) that is also​

 

 

 

present.​

 

See Section C.11.15.1.2.​

C.8.12.5.1 Optical Path Attribute Descriptions​

C.8.12.5.1.1 Optical Path Sequence and Optical Path Identifier​

The Optical Path Sequence (0048,0105) shall include an Item for every optical path used in the acquisition of the current image.​

For single-frame images that do not include the Multi-frame Functional Groups Module, this Sequence shall include only one Item.​

For images that use the Multi-frame Functional Groups Module, the optical path associated with each frame is identified by reference​ totheOpticalPathIdentifier(0048,0106)ofthisSequence(seeOpticalPathIdentificationSequence(0048,0207)inSectionC.8.12.6.2).​ The Optical Path Sequence may include Items for defined optical paths that are not referenced by any frame.​

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