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DICOM PS3.3 2020a - Information Object Definitions |
OVERVIEW |
Purpose of image is to provide an overview of the entire glass slide; shall include the label, the specimen |
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and the empty space that are shown in Figure C.8-16. |
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Note |
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One purpose of the OVERVIEW image is to allow visual quality control to assure that tissue |
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fragments have not been omitted when selecting areas (manually or automatically) to digitize at |
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high resolution. The OVERVIEW image is distinct from the lowest resolution VOLUME image(s) |
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of WSI pyramids. |
Value 4 (Derived Pixel) shall have the Defined Terms specified in Table C.8.12.4-3.
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Table C.8.12.4-3. Whole Slide Microscopy Image Derived Pixels |
NONE |
No derivation of pixels (original) |
RESAMPLED |
Pixels were derived by down sampling a higher resolution image |
No additional values shall be present.
C.8.12.4.1.2 Imaged Volume Width, Height, Depth
ThefullphysicalextentofthewholeslideimagetargetvolumeisdescribedintheAttributesImagedVolumeWidth(0048,0001),Imaged Volume Height (0048,0002), and Imaged Volume Depth (0048,0003). These values reflect the maximum extent in these dimensions. There is no requirement that this entire extent is actually encoded in frames of the Image SOP Instance, unless Dimension Organiz- ation Type (0020,9311) is present with a value of TILED_FULL.
The value of Imaged Volume Depth (0048,0003) shall not be 0. If only a single focal plane is imaged, the Imaged Volume Depth may be the optical depth of field as encoded in Slice Thickness (0018,0050) of the Pixel Measures Functional Group (see Sec- tion C.7.6.16.2.1).
C.8.12.4.1.3 Total Pixel Matrix Columns, Rows, Focal Planes
TotalPixelMatrixColumns(0048,0006),TotalPixelMatrixRows(0048,0007)andTotalPixelMatrixFocalPlanes(0048,0303)describe the size of the entire imaged volume as a single extent across all frames (tiles).
The extent would be as described in these Attributes if the whole volume would be imaged and encoded as a non-sparse pixel matrix with the pixel spacing as specified in Pixel Spacing (0028,0030) of the Pixel Measures Functional Group (see Section C.7.6.16.2.1), such as when Dimension Organization Type (0020,9311) is present with a value of TILED_FULL.
C.8.12.4.1.4 Total Pixel Matrix Origin Sequence and Image Orientation (Slide)
TotalPixelMatrixOriginSequence(0048,0008)specifiesthelocationofthetopleftmostpixelofthepixelmatrix,andImageOrientation (Slide) (0048,0102) specifies the direction cosines of the first row and the first column of the pixel matrix, both with respect to the Slide Coordinate System Frame of Reference (see Section C.8.12.2). Although the image acquisition may vary the true row and column orientation at the pixel scale to account for local variation in the physical specimen, this Attribute describes the orientation as if the Pixel Matrix were flat.
Note
Typically, Image Orientation (Slide) will describe only a planar rotation, as the image plane is usually nominally parallel to the slide surface.
C.8.12.4.1.5 Photometric Interpretation and Samples Per Pixel
See Section C.7.6.3.1.2.
Enumerated Values for Photometric Interpretation (0028,0004):
MONOCHROME2
RGB
YBR_FULL_422
YBR_ICT