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Page 926​

DICOM PS3.3 2020a - Information Object Definitions​

For Whole Slide Microscopy Image LOCALIZER images (see Section C.8.12.4.1.1 and Section C.8.12.7), all the optical paths asso-​ ciated with referenced images shall be described in Items of this Optical Path Sequence. The optical path associated with each refer-​ enced frame is identified in the Referenced Image Navigation Sequence (0048,0200) by reference to an Optical Path Identifier​ (0048,0106) of this Sequence. The Optical Path Identifiers used in the LOCALIZER image do not have to be identical to those used​ in the referenced images.​

Note​

1.​For example, each of four referenced images may use a different optical path (color), and within each of those image​ SOP Instances the single Optical Path Sequence (0048,0105) Item is identified as "1", although the meaning of optical​ path "1" is different for each image. The LOCALIZER, however, must include all four optical paths with distinct identifiers​ "1", "2", "3", and "4".​

2.​The Optical Path Identifier is not necessarily numeric; it is a string of up to 16 characters (VR SH).​

C.8.12.5.1.2 Illumination Type Code Sequence​

Illumination Type Code Sequence (0022,0016) specifies the type of illumination of the imaging target. This Attribute may include​ multiple Items, as some techniques may involve multiple methods, or some method identifiers may not be completely descriptive.​

Note​

For example, this Sequence might include two Items, (111741, DCM, "Transmission illumination") and (111748, DCM, "Dif-​ ferential interference contrast") to indicate "Transmission DIC".​

The type of illumination often prescribes the set of optical path components such as filters and prisms, and their arrangement in the​ optical path.​

Note​

For instance, phase contrast illumination will utilize a condenser annulus and a phase contrast plate; this may be indicated​ by an Item (111747, DCM, "Phase contrast illumination") in this Attribute, but with no further specification in the filters and​ lenses identification Attributes.​

C.8.12.5.1.3 Light Path and Image Path Filter Type Stack Code Sequences​

In some optical imaging methods, e.g., epifluorescence, there may be a single light path with its associated imaging components that​ is used in both illumination/excitation of the imaging target and in the transmitted/emitted path to the imaging sensor. In this case, the​ optical components are to be specified in the Image Path Filter Type Stack Code Sequence (0022,0018).​

C.8.12.5.1.4 ICC Profile​

ICCProfile(0028,2000)providesthetransformformappingstoredcolorpixelvaluesintoICCstandardcolorProfileConnectionSpace​ (PCS). For MONOCHROME2 pixels that have a preferred color rendering through the Palette Color Lookup Table Sequence​ (0048,0120),thisAttributeprovidesamappingofthatpreferredRGBcolorrenderingintoPCS.TheICCProfileshallfollowtherequire-​ ments specified in Section C.11.15.1.1.​

C.8.12.6 Whole Slide Microscopy Image Functional Group Macros​

The following sections contain Functional Group Macros specific to the VL Whole Slide Microscopy Image IOD.​

Note​

The Attribute descriptions in the Functional Group Macros are written as if they were applicable to a single frame (i.e., the​ Macro is part of the Per-frame Functional Groups Sequence). If an Attribute is applicable to all frames (i.e., the Macro is part​ of the Shared Functional Groups Sequence) the phrase "this frame" in the Attribute description shall be interpreted to mean​ "for all frames".​

C.8.12.6.1 Plane Position (Slide) Macro​

Table C.8.12.6.1-1 specifies the Attributes of the Plane Position (Slide) Functional Group Macro.​

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DICOM PS3.3 2020a - Information Object Definitions​ Page 927​

Table C.8.12.6.1-1. Plane Position (Slide) Macro Attributes​

Attribute Name​

Tag​

Type​

Attribute Description​

Plane Position (Slide) Sequence​

(0048,021A)​

1​ Describes position of frame in the Total Pixel Matrix and in the​

 

 

 

Slide Coordinate System Frame of Reference.​

 

 

 

Only a single Item shall be included in this Sequence.​

>Column Position In Total Image​

(0048,021E)​

1​ The column position of the top left hand pixel of the frame in the​

Pixel Matrix​

 

 

Total Pixel Matrix (see Section C.8.12.4.1.1). The column position​

 

 

 

of the top left pixel of the Total Pixel Matrix is 1.​

>RowPositionInTotalImagePixel​

(0048,021F)​

1​ The row position of the top left hand pixel of the frame in the Total​

Matrix​

 

 

Pixel Matrix (see Section C.8.12.4.1.1). The row position of the​

 

 

 

top left pixel of the Total Pixel Matrix is 1.​

>X Offset in Slide Coordinate​

(0040,072A)​

1​ TheXoffsetinmmfromtheOriginoftheSlideCoordinateSystem.​

System​

 

 

See Figure C.8-16.​

>Y Offset in Slide Coordinate​

(0040,073A)​

1​ TheYoffsetinmmfromtheOriginoftheSlideCoordinateSystem.​

System​

 

 

See Figure C.8-16.​

>Z Offset in Slide Coordinate​

(0040,074A)​

1​ The Z offset in µm from the Origin of the Slide Coordinate System,​

System​

 

 

nominally the surface of the glass slide substrate. See​

Figure C.8-17​

Note​

Required even if only a single focal plane was acquired.​

C.8.12.6.2 Optical Path Identification Macro​

Table C.8.12.6.2-1 specifies the Attributes of the Optical Path Identification Functional Group Macro.​

Table C.8.12.6.2-1. Optical Path Identification Macro Attributes​

Attribute Name​

Tag​

Type​ Attribute Description​

Optical Path Identification​

(0048,0207)​

1​

Identifies the optical path characteristics of this frame.​

Sequence​

 

 

Only a single Item shall be included in this Sequence.​

 

 

 

>Optical Path Identifier​

(0048,0106)​

1​

Uniquely identifies the path described in the Optical Path​

Sequence (0048,0105) by reference to an Item with the same​

Optical Path Identifier (0048,0106) value. See Section C.8.12.5.​

C.8.12.6.3 Specimen Reference Macro​

Table C.8.12.6.3-1 specifies the Attributes of the Specimen Reference Functional Group Macro. This allows association of the frame​ with specific specimens imaged on the same slide (e.g., tissue micro-arrays).​

Table C.8.12.6.3-1. Specimen Reference Macro Attributes​

Attribute Name​

Tag​

Type​

Attribute Description​

SpecimenReferenceSequence​

(0048,0110)​

2​

Identifies specimens that may be visible in this frame, and are​

 

 

 

fully identified in the Specimen Description Sequence​

 

 

 

(0040,0560).​

 

 

 

Zero or more Items shall be included in this Sequence.​

>Specimen UID​

(0040,0554)​

1​

Unique Identifier for Specimen. See Section C.7.6.22.1.2.​

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Page 928​

DICOM PS3.3 2020a - Information Object Definitions​

C.8.12.7 Multi-Resolution Navigation Module​

Table C.8.12.7-1 specifies the Attributes that describe the Multi-Resolution Navigation Module, used in Whole Slide Microscopy​ LOCALIZER images.​

Table C.8.12.7-1. Multi-Resolution Navigation Module Attributes​

Attribute Name​

Tag​

Type​

Attribute Description​

Referenced Image Navigation​

(0048,0200)​

1​

Identification of correspondence between areas of Pixel Data​

Sequence​

 

 

(07FE,0010) and frames of higher resolution images.​

 

 

 

One or more Items shall be included in this Sequence.​

>Include Table 10-11 “SOP Instance Reference Macro Attributes”​Referenced Image Instance​

>Referenced Frame Number​

(0008,1160)​

1​

Referenced Frame Number within Referenced Image. Only a​

 

 

 

single value shall be present.​

>Top Left Hand Corner of​

(0048,0201)​

1​

Top Left Hand Corner of Localizer image pixel area​

Localizer Area​

 

 

corresponding to referenced frame, specified as column\row.​

 

 

 

Column is the horizontal offset and row is the vertical offset.​

>Bottom Right Hand Corner of​

(0048,0202)​

1​

Bottom Right Hand Corner of Localizer image pixel area​

Localizer Area​

 

 

corresponding to referenced frame, specified as column\row.​

 

 

 

Column is the horizontal offset and row is the vertical offset.​

>Pixel Spacing​

(0028,0030)​

1​

Pixel Spacing in Referenced Image, specified as a numeric pair​

 

 

 

- adjacent row spacing \ adjacent column spacing, in mm.​

>Z Offset in Slide Coordinate​

(0040,074A)​

1​

The Z offset of the referenced frame from the Slide Coordinates​

System​

 

 

System reference plane, in microns​

>Samples per Pixel​

(0028,0002)​

1​

The number of samples (color planes) containing information in​

 

 

 

the referenced image.​

>Optical Path Identifier​

(0048,0106)​

1​

IdentifiestheopticalpathusedinacquiringthereferencedFrame​

 

 

 

by reference to an Item in the Optical Path Sequence​

 

 

 

(0048,0105)ofthecurrentSOPInstance.SeeSectionC.8.12.5.​

C.8.12.8 Slide Label Module​

Table C.8.12.8-1 specifies the Attributes that describe the interpretation of a scanned Slide Label.​

Table C.8.12.8-1. Slide Label Module Attributes​

Attribute Name​

Tag​

Type​

Attribute Description​

Barcode Value​

(2200,0005)​

2​

Barcode interpreted from the scanned slide label.​

 

 

 

Note​

 

 

 

This may be the same as Container Identifier​

 

 

 

(0040,0512).​

Label Text​

(2200,0002)​

2​

Labeltextinterpretedfromthescannedslidelabel,e.g.,byoptical​

 

 

 

character recognition.​

C.8.12.9 Whole Slide Microscopy Image Frame Type Macro​

Table C.8.12.9-1 specifies the Attributes of the Whole Slide Microscopy Image Frame Type Functional Group Macro.​

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DICOM PS3.3 2020a - Information Object Definitions​ Page 929​

Table C.8.12.9-1. Whole Slide Microscopy Image Frame Type Macro Attributes​

Attribute Name​

Tag​

Type​

Attribute Description​

Whole Slide Microscopy Image​

(0040,0710)​

1​ Identifies the characteristics of this Whole Slide Microscopy Image​

Frame Type Sequence​

 

 

frame.​

 

 

 

Only a single Item shall be included in this Sequence.​

>Frame Type​

(0008,9007)​

1​ Type of Frame. A multi-valued Attribute analogous to Image Type​

 

 

 

(0008,0008).​

Enumerated Values and Defined Terms are the same as those for​ thefourvaluesofImageType(0008,0008).SeeSectionC.8.12.4.1.​

C.8.12.10 VL Photographic Equipment Module​

Table C.8.12.10-1 specifies the Attributes that identify and describe a photographic device such as a camera.​

Table C.8.12.10-1. VL Photographic Equipment Module Attributes​

Attribute Name​

Tag​

Type​

Attribute Description​

Camera Owner Name​

(0016,004D)​

3​

The owner of the photographic equipment.​

Lens Specification​

(0016,004E)​

3​

Theminimumfocallength,maximumfocallength,minimum​

 

 

 

F number in the minimum focal length, and minimum F​

 

 

 

number in the maximum focal length for the lens that was​

 

 

 

used.​

Lens Make​

(0016,004F)​

3​

The lens manufacturer.​

Lens Model​

(0016,0050)​

3​

The lens's model name and model number.​

Lens Serial Number​

(0016,0051)​

3​

The serial number of the lens that was used.​

C.8.12.11 VL Photographic Acquisition Module​

Table C.8.12.11-1 specifies the Attributes that describe the process of acquisition of a photographic image.​

Table C.8.12.11-1. VL Photographic Acquisition Module Attributes​

Attribute Name​

Tag​

Type​

Attribute Description​

White Point​

(0016,0001)​

3​ The chromaticity of the white point of the image. This is the chromaticity​

 

 

 

when each of the primaries has its reference white value. The value is​

 

 

 

described using the 1931 CIE xy chromaticity diagram.​

Primary Chromaticities​

(0016,0002)​

3​ The chromaticity of the three primary colors of the image. This is the​

 

 

 

chromaticity for each of the primaries when it has its reference white​

 

 

 

value and the other primaries have their reference black values. These​

 

 

 

values are described using the 1931 CIE xy chromaticity diagram.​

Battery Level​

(0016,0003)​

3​ A description of the camera’s battery level at the time of image capture,​

 

 

 

encoded as a decimal, ratio, or text string.​

ExposureTimeinSeconds​

(0016,0004)​

3​ Exposure time in seconds.​

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Page 930​

DICOM PS3.3 2020a - Information Object Definitions​

Attribute Name​

Tag​

Type​

Attribute Description​

F-Number​

(0016,0005)​

3​

The f-number.​

 

 

 

Note​

 

 

 

1.​The nomenclature for representing the f-number varies in​

 

 

 

the literature. The capitalized hyphenated form is used​

 

 

 

heretobeconsistentwiththepatternusedforotherDICOM​

 

 

 

Attribute names.​

 

 

 

2.​The f-number is defined as the ratio of the focal length to​

 

 

 

the diameter of the entrance pupil.​

OECF Rows​

(0016,0006)​

3​ ThenumberofrowsintheOpto-ElectronicConversionFunction(OECF),​

 

 

 

as specified in [ISO 14524].​

OECF Columns​

(0016,0007)​

3​ The number of columns in the Opto-Electronic Conversion Function​

 

 

 

(OECF), as specified in [ISO 14524].​

OECF Column Names​

(0016,0008)​

3​ The names of the columns in the Opto-Electronic Conversion Function​

 

 

 

(OECF), as specified in [ISO 14524].​

OECF Values​

(0016,0009)​

3​ The values of the Opto-Electronic Conversion Function (OECF), as​

 

 

 

specified in [ISO 14524], encoded as successive column values of the​

 

 

 

first row, then as successive column values of each successive row.​

Spatial Frequency​

(0016,000A)​

3​ The number of rows in the Spatial Frequency Response (SRF) table as​

Response Rows​

 

 

specified in [ISO 12233].​

Spatial Frequency​

(0016,000B)​

3​ The number of columns in the Spatial Frequency Response (SRF) table​

Response Columns​

 

 

as specified in [ISO 12233].​

Spatial Frequency​

(0016,000C)​

3​ The names of the columns in the Spatial Frequency Response (SRF)​

Response Column Names​

 

 

table as specified in [ISO 12233].​

Spatial Frequency​

(0016,000D)​

3​ The values of the Spatial Frequency Response (SRF) table as specified​

Response Values​

 

 

in [ISO 12233], encoded as successive column values of the first row,​

 

 

 

then as successive column values of each successive row.​

Color Filter Array Pattern​

(0016,000E)​

3​ The number of rows in the color filter array (CFA) geometric pattern.​

Rows​

 

 

 

Color Filter Array Pattern​

(0016,000F)​

3​ The number of columns in the the color filter array (CFA) geometric​

Columns​

 

 

pattern.​

Color Filter Array Pattern​

(0016,0010)​

3​ The values of the color filter array (CFA) geometric pattern of the image​

Values​

 

 

sensor when a one-chip color area sensor is used, encoded as​

 

 

 

successive column values of the first row, then as successive column​

 

 

 

values of each successive row.​

Exposure Program​

(0016,0016)​

3​ The class of the program used by the camera to set exposure.​

 

 

 

Defined Terms:​

0​Not defined​ 1​Manual​ 2​Normal program​ 3​Aperture priority​ 4​Shutter priority​

5​Creative program (biased toward depth of field)​ 6​Action program (biased toward fast shutter speed)​

7​Portrait mode (for closeup photos with the background out of focus)​ 8​Landscapemode(forlandscapephotoswiththebackgroundinfocus)​

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