Материал: b823180j

On a final note, there is more cell elongation and actin filament

3

J. E. Moore, Jr., E. Burki, A. Suciu, S. Zhao, M. Burnier,

alignment in the direction of flow at higher shear levels (Fig. 3

H. R. Brunner and J. J. Meister, Ann. Biomed. Eng., 1994, 22, 416.

4

S. Usami, H. H. Chen, Y. Zhao, S. Chien and R. Skalak, Ann. Biomed.

and Fig. 6C). This agrees with findings in a number of studies.22,25

Eng., 1993, 21, 77.

In addition, in a small 2 h perfusion study, the cells were observed

5

C. Urbich, E. Dernbach, A. Reissner, M. Vasa, A. M. Zeiher and

to migrate downstream in the direction of flow. Future studies

S. Dimmeler, Arterioscler. Thromb. Vasc. Biol., 2002, 22, 69.

will incorporate

time lapse microscopy to investigate cell

6

J. Seebach, P. Dieterich, F. Luo, H. Schillers, D. Vestweber,

H. Oberleithner, H. J. Galla and H. J. Schnittler, Lab Invest., 2000,

behaviour with shear over time.

80, 1819.

7

M. Morigi, C. Zoja, M. Figliuzzi, M. Foppolo, G. Micheletti,

Conclusions

M. Bontempelli, M. Saronni, G. Remuzzi and A. Remuzzi, Blood,

1995, 85, 1696.

This multishear

microdevice delivers

physiologically

relevant

8

C. G. Galbraith, R. Skalak and S. Chien, Cell Motil. Cytoskeleton,

shear stresses, spanning over two orders of magnitude for any

1998, 40, 317.

9

J. Y. Ji, H. Jing and S. L. Diamond, Circ. Res., 2003, 92, 279.

one flow rate, making it a very convenient and time saving tool

10

E. Gutierrez, B. G. Petrich, S. J. Shattil, M. H. Ginsberg,

for mapping or screening cell behaviour. In addition, it requires

A. Groisman and A. Kasirer-Friede, Lab Chip, 2008, 8, 1486.

minimal

sample

volume,

making

it

attractive

for

studies

11

D. P. Gaver, 3rd and S. M. Kute, Biophys. J., 1998, 75, 721.

12

G. Kaplanski, C. Farnarier, A. M. Benoliel, C. Foa, S. Kaplanski and

involving

scarce

and expensive samples. To date,

this device

P. Bongrand, J. Cell Sci., 1994, 107(Pt 9), 2449.

covers the widest range of shear stresses in comparison to other

13

W. Van Driessche, P. De Smet and G. Raskin, Pfluegers Arch., 1993,

published shear devices. We used it to study HUVECs and our

425, 164.

results showed that there was a shear-dependent regulation of

14

E. B. Hunziker and R. K. Schenk, J. Physiol., 1989, 414, 55.

15

H. G. Verhage, M. L. Bareither, R. C. Jaffe and M. Akbar, Am.

vWF, and cell elongation and actin filament alignment in the

J. Anat., 1979, 156, 505.

direction of shear.

16

A. M. Malek, S. L. Alper and S. Izumo, Jama, 1999, 282, 2035.

17

A. Gnasso, C. Carallo, C. Irace, M. S. De Franceschi, P. L. Mattioli,

Acknowledgements

C. Motti and C. Cortese, Atherosclerosis, 2001, 156, 171.

18

H. Lu, L. Y. Koo, W. M. Wang, D. A. Lauffenburger, L. G. Griffith

The authors wish to acknowledge Dr Nicholas Timmins and

and K. F. Jensen, Anal. Chem., 2004, 76, 5257.

19

G. M. Walker, H. C. Zeringue and D. J. Beebe, Lab Chip, 2004, 4, 91.

Dr Ian Aird from the Australian Institute for Bioengineering and

20

P. Feugier, R. A. Black, J. A. Hunt and T. V. How, Biomaterials,

Nanotechnology (AIBN) for donating HUVECs, and funding

2005, 26, 1457.

from Australian

Research

Council

(ARC) Discovery

Grants

21

M. Galbusera, C. Zoja, R. Donadelli, S. Paris, M. Morigi, A. Benigni,

M. Figliuzzi, G. Remuzzi and A. Remuzzi, Blood, 1997, 90, 1558.

Scheme.

22

R. J. Sun, S. Muller, X. Wang, F. Y. Zhuang and J. F. Stoltz, Clin.

Hemorheol. Microcirc., 2000, 23, 1.

References

23

G. Hacker, Cell Tissue Res., 2000, 301, 5.

24

B. Levkau, B. Herren, H. Koyama, R. Ross and E. W. Raines, J. Exp.

1 D. M. Wootton and D. N. Ku, Annu. Rev. Biomed. Eng., 1999, 1, 299.

Med., 1998, 187, 579.

2 P. F. Davies, C. F. Dewey, Jr., S. R. Bussolari, E. J. Gordon and

25

C. F. Dewey, Jr., S. R. Bussolari, M. A. Gimbrone, Jr. and

M. A. Gimbrone, Jr., J. Clin. Invest., 1984, 73, 1121.

P. F. Davies, J. Biomech. Eng., 1981, 103, 177.